C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C07H 21/04 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01) C12N 15/00 (2006.01) C12N 15/09 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2406939
The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
La présente invention concerne la biologie moléculaire et cellulaire. L'invention concerne de manière générale les enzymes transcriptases inverses et des méthodes de transcription inverse de molécules d'acide nucléique, en particulier des molécules d'ARN messager. L'invention concerne, en particulier, des enzymes transcriptases inverses mutées ou modifiées afin d'accroître leur thermostabilité, réduire leur activité de transférase terminale, et/ou accroître leur fidélité, ainsi que des méthodes de production, d'amplification ou de séquençage des molécules d'acide nucléique (en particulier des molécules d'ADN complémentaire) à l'aide de ces enzymes transcriptases inverses ou compositions. L'invention concerne également des molécules d'acide nucléique obtenues selon ces méthodes et l'utilisation de ces molécules d'acide nucléique pour obtenir des polypeptides désirés. L'invention concerne enfin des kits comprenant ces enzymes ou compositions.
Dhariwal Gulshan
Gerard Gary F.
Potter Robert Jason
Rosenthal Kim
Smith Michael D.
Invitrogen Corporation
Life Technologies Corporation
Mbm Intellectual Property Law Llp
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