Enzyme/tag binding and detection system

Details Enzyme/tag binding and detection system Enzyme/tag binding and detection system

C - Chemistry, Metallurgy

07

K

C07K 17/00 (2006.01) C07K 1/36 (2006.01) C07K 14/81 (2006.01) C12N 9/00 (2006.01) C12N 9/10 (2006.01) C12N 9/50 (2006.01) C12N 11/00 (2006.01)

Patent

CA 2390522

A hybrid polypeptide composed of an identification peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is con- strutted that includes segments of DNA coding for the identification peptide and the de- sired functional protein. The identification peptide consists of a specific sequence of amino acids that has the desired affinity for a non-antibody capture protein. This peptide maybe linked to either the amino or carboxyl terminal of the functional protein. The identification peptide may or may not contain a sequence of amino acids that can be fragmented by se- quence specific proteases or chemical agents to yield the native protein. The hybrid poly- peptide produced by either cell or cell-free based expression systems is now suitable for further processing: This fusion protein can be purified by affinity chromatographic tech- piques using an immobilized non-antibody capture protein that has the desired affinity for the identification peptide: Alternately the capture protein may contain a label that will allow for the tracking of the functional protein in the system being studied.

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