C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/00 (2006.01) C09K 3/00 (2006.01) C09K 11/07 (2006.01) C12Q 1/28 (2006.01) C12Q 1/68 (2006.01) G01N 21/76 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2300096
Methods and compositions for generating chemiluminescence on reaction with a peroxidase enzyme are provided as well as novel compounds useful therein. The compounds comprise a C-C double bond substituted at one carbon with two oxygen or sulfur atom-containing groups. The compositions comprise the double bond containing compound, a peroxide and optionally a peroxidase activity enhancing substance in an aqueous solution. The compositions can additionally comprise a nonionic surfactant or a cationic surfactant or both to improve detection sensitivity of the peroxidase. The novel chemiluminescent methods and compositions are useful in assays for peroxidase enzymes and in assays employing enzyme-labeled specific binding pairs.
L'invention porte sur des méthodes et compositions génératrices de chimioluminescence par réaction avec l'enzyme peroxydase et sur de nouveaux composés à cet effet. Lesdits composés consistent en une liaison double C-C substituée en l'un des C par des groupes contenant deux atomes d'oxygène ou atomes de soufre. Les compositions comprennent le susdit composé à liaison double, un peroxyde, et facultativement une substance renforçant l'activité de l'oxydase en solution aqueuse; elles peuvent de plus contenir un tensio-actif non ionique, cationique ou les deux, accroissant la sensibilité de détection de la peroxydase. Lesdites méthodes et compositions génératrices de chimioluminescence servent au dosage des peroxydases et à des dosages utilisant des paires de liaisons spécifiques marquées par des enzymes.
Gowling Lafleur Henderson Llp
Lumigen Inc.
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