Improved transformation method for plants

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) C12N 5/04 (2006.01)

Patent

CA 2252612

A process for integrating a DNA fragment into the genome of a cell of a monocotyledonous plant, the process comprising the steps of: 1) incubating, prior to contacting with the DNA fragment, a culture of untransformed monocotyledonous plant cells on a medium comprising a plant phenolic compound, for a period of time sufficient to stimulate cell division and enhance competence for integration of foreign DNA; and 2) contacting the untransformed cells with the DNA fragment under conditions in which the DNA fragment is taken up by the untransformed cells and is stably integrated in the genome of the untransformed cells, to generate transformed cells.

L'invention concerne un procédé pour intégrer un fragment d'ADN dans le génome d'une cellule d'un végétal monocotylédone, ce procédé consistant à: 1) incuber, avant mise en contact avec le fragment d'ADN, une culture de cellules végétales monocotylédones non transformées sur un milieu comprenant un composé phénolique végétal, pendant une durée suffisante pour stimuler la division cellulaire et amplifier la compétence pour l'intégration d'un ADN étranger; et 2) mettre en contact les cellules non transformées avec le fragment dans des conditions dans lesquelles ledit fragment est absorbé par les cellules non transformées et est intégré de manière stable dans le génome des cellules non transformées, en vue de générer des cellules transformées.

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