Method of constructing cdna tag for identifying expressed...

Details Method of constructing cdna tag for identifying expressed... Method of constructing cdna tag for identifying expressed...

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2455354

A cDNA tag for identifying an expressed gene is constructed by combining two types of linkers X and Y containing a II type restriction enzyme, two IIS type restriction enzymes and the recognition sequence of one of the IIS type restriction enzymes. This cDNA tag is usable in analyzing an expressed gene either as such or after a ligation treatment or the like.

La présente invention concerne un marqueur d'ADNc servant à identifier un gène exprimé, ledit marqueur étant construit grâce à la combinaison de deux types d'agents de liaison X et Y contenant une enzyme de restriction de type II, deux enzymes de restriction de type IIS, et la séquence de reconnaissance de l'une des enzymes de restriction de type IIS. Ce marqueur d'ADNc peut être utilisé pour l'analyse d'un gène exprimé, soit tel quel, soit après une opération de ligature ou analogue.

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