Method for specifically detecting a coagulation factor v...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/56 (2006.01) C12Q 1/37 (2006.01) G01N 33/86 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2162531

The invention relates to a method for qualitative detection and quantitative determination of the stability of activated coagulation factor V towards proteolytic degradation in a biological fluid sample, which includes mixing the sample with reagent A whose content of functional factor V is diminished as compared with normal, human plasma, adding a reagent B for activating the factor V of the sample, adding a reagent C for proteolytically degrading the activated factor V of the sample and adding reagents for determining residual factor Va activity. The sample forms at most 20% (pref. <= 10%) of the total test vol.

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