Method of detecting an analyte using a holographic sensor

G - Physics – 01 – N

Patent

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G01N 21/47 (2006.01) G03H 1/02 (2006.01)

Patent

CA 2569540

A method for the detection of an analyte in a fluid, which comprises contacting the fluid with a holographic element comprising a medium and a hologram disposed throughout the volume of the medium, wherein an optical characteristic of the element changes as a result of a variation of a physical property occurring throughout the volume of the medium, and wherein the variation arises as a result of interaction between the medium and the analyte; and detecting any change of the optical characteristic of the element; wherein (a) the medium comprises a group which is capable of reacting with the analyte, wherein the analyte or the group is capable of existing in a plurality of forms, and the detecting is conducted in the presence of a first catalyst which is capable of catalysing the conversion of a relatively less reactive form of the analyte or group to a relatively more reactive form; or (b) the fluid comprises a component, other than the analyte, which is capable of interacting with the medium, and the detecting is conducted in the presence of a second catalyst capable of catalysing the removal of said component.

L'invention concerne un procédé qui permet de détecter un analyte dans un liquide, lequel procédé consiste: à mettre en contact le liquide avec un élément holographique comprenant un support et un hologramme disposé dans le volume du support, une caractéristique optique de l'élément changeant en fonction d'une variation d'une propriété physique s'exerçant dans tout le volume du support, ladite variation se produisant à la suite d'une interaction entre le support et l'analyte; et à détecter un quelconque changement de la caractéristique optique de l'élément; (a) ledit support comprenant un groupe capable de réagir avec l'analyte, l'analyte ou le groupe étant capable d'exister sous une pluralité de formes, et la détection étant réalisée en présence d'un premier catalyseur capable de catalyser la conversion d'une forme relativement moins réactive de l'analyte ou du groupe en une forme relativement plus réactive; ou (b) le liquide comprenant un composant, autre que l'analyte, capable d'interagir avec le support, et la détection étant réalisée en présence d'un second catalyseur capable de catalyser l'élimination dudit composant.

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