Non-fluorescent energy transfer

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01)

Patent

CA 2624917

The present invention relates generally to the transfer of non-fluorescent energy between donor and acceptor moieties. In certain embodiments, the invention provides biomolecules that include substantially non-fluorescent donor moieties. Processes involving these donor moieties typically entail reduced background fluorescence relative to applications that involve conventional fluorescent donor moieties. In addition to reaction mixtures and methods that include the use of these biomolecules, the invention also provides related kits and systems.

La présente invention concerne de manière générale le transfert d'énergie non fluorescente entre des groupes donneurs et des groupes accepteurs. Dans certains modes de réalisation, l'invention concerne des biomolécules qui comprennent des groupes donneurs sensiblement non fluorescents. Des processus impliquant ces groupes donneurs donnent lieu en général à une fluorescence d'arrière-plan limitée par rapport à des applications qui impliquent des groupes donneurs fluorescents conventionnels. En plus de mélanges réactionnels et de procédés qui comprennent l'utilisation de ces biomolécules, l'invention a également pour objet des kits et systèmes correspondants.

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