Maize yellow stripe1 and related genes

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) C07K 14/415 (2006.01)

Patent

CA 2429142

The maize yellow stripe1 gene encodes a transmembrane protein which mediates the uptake of chelated iron from soil. The gene is shown here to be upregulated, and protein production increased, under conditions of low iron bioavailability. The protein is also shown to be able to transport other metals as well, such as copper. The present invention also provides a family of Arabidopsis genes and gene products related to ys1 termed here as ys11-8. The invention provides the genes and their gene products as well as methods to create and use transgenic plants for the increased uptake of iron in nutritional crops as well as for the bioremediation of soil.

L'invention concerne le gène yellow stripe 1 (ys 1) du maïs, qui code une protéine transmembranaire assurant la médiation de l'absorption de fer chélaté depuis le sol. Le gène apparaît ici comme bénéficiant d'une régulation positive, et la production de protéine augmente, dans des conditions de faible biodisponibilité du fer. Il apparaît en outre que la protéine est capable de transporter également d'autres métaux, comme le cuivre. L'invention concerne par ailleurs une famille de gènes Arabidopsis et de produits géniques associés au gène ys1 que l'on appelle ys11-8. L'invention concerne les gènes et leurs produits géniques ainsi que des procédés relatifs à la création et à l'utilisation de plantes transgéniques permettant d'augmenter l'absorption du fer, dans les cultures à vocation nutritive, et d'assurer la biorestauration du sol.

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