Method for evaluating the extent of a lithogenesis process...

G - Physics – 01 – N

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G01N 33/493 (2006.01) G01N 25/14 (2006.01)

Patent

CA 2136195

The present method for evaluating the extent of a lithogenesis process intensity and for determining the composition of lithogenic urate salts in urolithiasis resides in adding an aqueous protein solution to a urine sample in a ratio of 9:1, 7:1 or 5:1, respectively; applying the mixture thus-prepared as a drop onto a smooth surface for drying for at least 24 hours; determining the nature of crystallization of urate salts in the marginal zone of the urine sample, the presence of separate, singly occurring crystals, conglomerates of crystals and a complete crystallization of this marginal zone being indicative, respectively, of a weak, moderate or a highly pronounced extent of a lithogenesis process intensity; the composition of crystalline formations is determined, while, simultaneously, establishing the constitution of urate salts in the central zone of that same urine sample, followed by carrying out a comparative analysis to said two compositions to determine the composition of lithogenic urate salts.

La présente méthode porte sur l'évaluation de l'ampleur du processus de lithogenèse et sur l'établissement de la composition des sels uriques lithogènes dans les résidus de l'urolithiase par addition d'une solution aqueuse de protéine à un échantillon d'urine dans un rapport respectivement de 9:1, 7:1 ou 5:1; l'application du mélange ainsi préparé sous forme de goutte sur une surface lisse pour séchage pendant au moins 24 heures; la détermination de la nature de la cristallisation des sels uriques dans la zone marginale de l'échantillon d'urine, la présence de cristaux distincts seuls, de groupes de cristaux et d'une cristallisation complète de cette zone marginale indicatrice, respectivement de l'ampleur faible, moyenne ou très prononcée de la lithogenèse; la composition de formations cristallines est déterminée, tout en établissant simultanément la constitution de sels uriques dans la zone centrale du même échantillon d'urine, suivie d'une analyse comparative des deux compositions afin d'établir la composition des sels uriques lithogènes.

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