Production of radial glial cells

Details Production of radial glial cells Production of radial glial cells

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12N 5/0797 (2010.01) A61K 35/12 (2006.01) A61K 35/30 (2006.01) A61K 48/00 (2006.01) A61P 25/28 (2006.01) A61K 38/18 (2006.01) A61K 38/19 (2006.01)

Patent

CA 2364095

The present invention relates to a method of producing radial glial cells from neural stem cells, particularly by contacting neural stem cells with epidermal growth factor (EGF), fibroblast growth factor 2 (FGF-2) and/or TGF.alpha.. Leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) can optionally be added to enhance the effect of EGF, FGF-1 or TGF.alpha..

La présente invention porte sur une méthode permettant de produire des cellules gliales radiales à partir de cellules souches neuronales, en particulier, en mettant en contact des cellules souches neuronales avec de l'EGF (epidermal growth factor, du FGF-2 (fibroblast growth factor 2) et/ou du TGF alpha. Du LIF (leukemia inhibitory factor) et du CNTF (ciliary neurotrophic factor) peuvent aussi être ajoutés pour augmenter l'effet de l'EGF, du FGF-1 ou du TGF alpha.

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