Protein from lactic acid bacteria

Details Protein from lactic acid bacteria Protein from lactic acid bacteria

C - Chemistry, Metallurgy

12

N

C12N 15/57 (2006.01) A23C 19/032 (2006.01) A23C 19/055 (2006.01) A23C 19/06 (2006.01) A23J 1/00 (2006.01) A61K 39/395 (2006.01) C12N 1/21 (2006.01) C12N 9/52 (2006.01) C12N 15/65 (2006.01) C12P 21/08 (2006.01) G01N 33/573 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2068811

Title: protein from lactic acid bacteria ABSTRACT The present invention relates to an endopeptidase of lactic acid bacteria in isolated or purified form as well as to uses thereof, e.g., in the preparation of foods such as cheese, whippable products and meat products, or for preparing antibodies against the endopeptidase. The invention also relates to recombinant genetic information coding for the endopeptidase and transformed organisms expressing the endopeptidase. The endopeptidase can be isolated from Lactococcus lactis subsp. cremoris Wg2. It has a molecular weight of 70 kD ? 5 kD; an isoelectric point of 4.1 ? 0.4; a substrate range which includes peptides having five or more amino acids, metenkaphalin, bradykinin, glucagon, oxidized insulin .beta.-chain and neurotensin, and excludes peptides having less than 5 amino acids, and .alpha.-, .beta.- and ?-casein; a hydrolysis activity dependent of Co2+; a temperature optimum at 30-38°C and a pH optimum at 6.0-6.5.

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