Method for induction of apoptotic cell death in malignant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01) C12N 15/85 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2283090

The present invention relates to use of a vector containing genetic material for the preparation of a composition for the treatment of malignant disease, said vector, upon administration in a subject suffering from malignant disease, inducing apoptosis in malignant cells by reducing the ratio of the level of pRb protein to the level of an apoptosis-inducing protein in malignant cells, by reducing the level of pRb and increasing the level of the apoptosis-inducing protein, after having first achieved growth arrest of said malignant cells by inducing herein an inhibition of phosphorylation of the pRb protein. The invention further relates to specific vectors for efficient transfer.

Cette invention porte sur l'utilisation d'un vecteur renfermant un matériel génétique aux fins de la préparation d'une composition de traitement d'affections malignes. Administré à un sujet atteint d'une affection maligne, ce vecteur provoque l'apoptose des cellules malignes par réduction du rapport teneur en protéine pRb/teneur en protéine induisant l'apoptose ainsi que par réduction de la teneur en protéine pRb et élévation de la teneur en protéine induisant l'apoptose et, une fois obtenue la cessation de la croissance desdites cellules malignes, il provoque une inhibition de la phosphorylisation de la protéine pRb. Cette invention concerne également des vecteurs spécifiques aux fins d'un transfert efficace.

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