Purified porcine kidney l-fucose kinase

Details Purified porcine kidney l-fucose kinase Purified porcine kidney l-fucose kinase

C - Chemistry, Metallurgy

12

N

C12N 9/12 (2006.01)

Patent

CA 2331276

The present invention provides an enzyme L-fucokinase isolated from porcine kidney and in homogeneously purified form. The enzyme has a native molecular weight of 440 kDa based on gel filtration, a subunit molecular weight of about 110 kDa based on SDS PAGE, an optimal pH of about 8.0, and wherein said enzyme catalyzes the phosphorylation of L-fucose and does not phosphorylate D- glucose, D-galactose and D-mannose.

La présente invention concerne une enzyme L-fucokinase isolée à partir de rein de porc, et se présentant sous une forme purifiée de façon homogène. L'enzyme présente un poids moléculaire natif de 440 kDa sur la base d'une filtration sur gel, un poids moléculaire de sous-unités d'environ 110kDa sur la base d'une électrophorèse sur gel de polyacrylamide en présence de SDS, un pH optimal d'environ 8,0, ladite enzyme catalysant la phosphorylation de L-fucose sans phosphoryler la D-glucose, la D-galactose et la D-mannose.

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