Recombinant expression vectors for functional nav1.9 sodium...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/10 (2006.01) C07K 14/705 (2006.01) C12N 15/63 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2479158

The present invention is drawn to the stable cloning and expression of Nav1.9 sodium channel at the mRNA and protein levels, production of sodium channel currents characteristic of native currents in dorsal root ganglion neurons and to cell lines transiently or stably expressing recombinant Nav1.9 sodium channel.

L'invention concerne le clonage stable et l'expression d'un canal sodium Na¿v?1.9 aux niveaux de l'ARNm et de la protéine; la production de courants par le canal sodium caractéristiques des courants d'origine dans les neurones des ganglions de la racine dorsale. L'invention concerne également des lignées cellulaires exprimant de manière stable et transitoire un canal sodium Na¿v?1.9.

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