C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/89 (2006.01) A01K 67/027 (2006.01) A61K 38/18 (2006.01) A61K 38/19 (2006.01) C07K 14/47 (2006.01) C07K 14/50 (2006.01) C07K 14/54 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/87 (2006.01) C12Q 1/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)
Patent
CA 2602876
We plan on injecting a tube into healthy fed pigs (eg. Omega 3; Antioxidants) pigs' and/or cows' and/or elephants' and/or any large easy to impregnate and frequent pregnancies to drain some of the flow of amniotic fluid from the umbilical chord/placenta to create tissue culture medium for stem cells of any type. We are also looking at taking DNA from the cells in the gonads of a healthy adult and apply electricity into an Oocyte with its nucleus removed and follow regular tissue culture stem cell/cloning procedures. Furthermore we could take the stem cells that continue to proliferate the longest and micro inject their DNA into female Oocytes and apply electricity... Re: All Animals; eg. pigs, cows, elephants, rhinos, alligator/crocodile, sturgeon, Boa Constrictor/Anaconda/Python, Tigers, Lions, Mountain Lions, (crickets, locusts - easy to multiply in very short period of time, pigeons, chickens, Lama, Emu, Ostriches, Caribou, Moose, Deer, Elks, Whales, Sharks... We want either larger cells or more cell proliferations to be used as food source or feeder layer for cloning eg. human stem cells, or used for in vitro; in vivo cloning a specimen with desirable phenotypes. We are also trying to use different levels of LIF (leukemia inhibitory factor) to trigger STAT 3. As well we are experimenting the gene POU5f1 gene that encodes the Oct - 4. We plan separate the and replate the colonies to form single wells. Additionally we will experiment with basic fibroblast growth factor (bFGF) and forsklin. We also plan as stated in our past patents use telomerase enzymes (such as [1, 36]). Furthermore: 1. We could take the cells that are still undifferentiated and continuing to proliferate and replating to start new colonies. 2. We could combine this with our own patented method/technique of taking stems cells that have finished proliferation and re injecting their DNA(s) into mass numbers Oocyte (with their nucleus removed).
Na
Voon Gerard
LandOfFree
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