Fungal isolates and biological control compositions for the...

A - Human Necessities – 01 – N

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A01N 63/04 (2006.01) A01H 5/10 (2006.01) A01P 13/00 (2006.01) C12N 1/14 (2006.01) C12P 19/34 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2616152

The present invention discloses fungal isolates of Phoma macrostoma or extracts obtained therefrom, useful for the control of broad leaf weeds, including Canada thistle, perennial sowthistle, dandelion, scentless chamomile, false cleavers, chickweed, wild buckwheat, and field bindweed. The present invention also discloses biological control compositions comprising fungal isolates formulated in a growth medium for maintaining the viability of the fungal isolates when the biological control composition is applied to soil. The present invention also discloses a novel probe and primer pair sequence for use in detecting Phoma macrostoma isolates that exhibit biocontrol activity. The present invention also discloses methods of screening fungal isolates to determine if they exhibit biocontrol activity.

L'invention concerne des isolats fongiques de Phoma macrostoma ou des extraits obtenus à partir de ceux-ci, utiles pour lutter contre la mauvaise herbe à feuilles larges, notamment le chardon des champs, le laiteron vivace, le pissenlit, la matricaire inodore, le gaillet gratteron, le mouron des oiseaux, la Vrillée batarde, et le liseron des champs. L'invention concerne également des compositions de lutte biologique comprenant des isolats fongiques préparés dans un milieu de croissance afin de conserver la viabilité des isolats fongiques lorsque l'on applique la composition de lutte biologique sur le sol. L'invention concerne en outre une nouvelle sonde et une séquence de paire d'amorces servant à détecter des isolats de Phoma macrostoma qui présentent une activité de lutte biologique. L'invention concerne enfin des procédés de criblage d'isolats fongiques permettant de déterminer s'ils présentent une activité de lutte biologique.

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