Production of recombinant igm in per.c6 cells

Details Production of recombinant igm in per.c6 cells Production of recombinant igm in per.c6 cells

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C07K 16/00 (2006.01) C07K 16/30 (2006.01) C12N 15/00 (2006.01) C12N 15/13 (2006.01) C12N 15/85 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2521490

The invention provides an immortalized human retina cell, per.C6 TM expressing E1A and E1B proteins of an adenovirus, wherein said cell comprises recombinant nucleic acid encoding an IgM molecule. The invention also provides a method for recombinant production of an IgM molecule, said method comprising culturing a cell according to the invention and expressing said recombinant nucleic acid encoding an IgM.

L'invention concerne une cellule de rétine humaine immortalisée, PER.C6 <TM> exprimant des protéines E1A et E1B d'un adénovirus, la cellule comprenant un acide nucléique recombinant codant une molécule IgM. L'invention concerne également un procédé de production par recombinaison d'une molécule IgM consistant à cultiver une cellule selon l'invention et à exprimer l'acide nucléique recombinant codant une IgM.

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