Genes of the dead box protein family, their expression...

Details Genes of the dead box protein family, their expression... Genes of the dead box protein family, their expression...

C - Chemistry, Metallurgy

12

N

C12N 15/61 (2006.01) A61K 31/713 (2006.01) A61K 38/52 (2006.01) C07K 16/40 (2006.01) C12N 9/90 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2335646

The invention relates to the preparation of two novel nucleic acids -Hc1 and Hc2- which are homologous to known DEAD box proteins, which correspond to RNA helicases, and which can be obtained from ciliates. The invention also relates to the insertion of the novel nucleic acids into suited target cells using recombinant DNA technologies, and to the use thereof for regulating transcription and translation. In addition, the invention relates to the in- vitro or in-vivo transcription of the novel nucleic acids for producing novel proteins and to the use thereof in treatment and diagnosis.

La présente invention concerne la préparation de deux nouveaux acides nucléiques (Hc1 et Hc2) qui constituent des homologues de protéines de la boîte DEAD connues, correspondent aux ARN hélicases et peuvent être obtenus à partir de ciliés. L'invention concerne également l'introduction des nouveaux acides nucléiques, selon des technologies de recombinaison d'ADN, dans des cellules cibles appropriées, et leur utilisation pour la régulation de la transcription et de la traduction. L'invention concerne en outre la transcription in vitro ou in vivo des nouveaux acides nucléiques pour la production de nouvelles protéines, ainsi que leur utilisation pour la thérapie et le diagnostic.

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