Method of measuring molecular interactions

Details Method of measuring molecular interactions Method of measuring molecular interactions

G - Physics

01

N

G01N 21/64 (2006.01) G01J 3/44 (2006.01) G01J 3/457 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2493993

The invention features a method of assaying for the interaction of a probe and an unknown target, said method including a) exciting a sample with radiation, the sample including at least one unknown target, at least one probe, and at least one fluorescent tag, b) measuring the fluorescence from a subvolume of the sample, and c) analyzing the fluctuations of the fluorescence.

L'invention concerne un procédé permettant de mesurer l'interaction d'une sonde et d'une cible inconnue. Ce procédé consiste : (a) à exciter un échantillon au moyen d'un rayonnement, lequel échantillon comprend au moins une cible inconnue, au moins une sonde et au moins un marqueur fluorescent ; (b) à mesurer la fluorescence à partir d'un sous-volume de l'échantillon ; et (c) à analyser les variations de la fluorescence.

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