Hypoallergenic proteins

G - Physics – 01 – N

Patent

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G01N 33/68 (2006.01)

Patent

CA 2678170

The present invention relates to a method for identifying a hypoallergenic derivative of a wild-type allergen comprising: determining the three-dimensional structure of a wild-type allergen; introducing at least one point mutation into said wild-type allergen, thereby obtaining a mutated allergen; identifying a hypoallergenic derivative of said wild-type allergen by detecting a destabilisation or change of the three-dimensional structure of the mutated allergen compared to the wild-type allergen by determining energy differences between the wild-type allergen and the mutant allergen expressed as a Z-score.

La présente invention concerne un procédé d'identification d'un dérivé hypoallergénique d'un allergène de type sauvage, consistant à déterminer la structure tridimensionnelle d'un allergène de type sauvage; à introduire au moins une mutation ponctuelle dans ledit allergène de type sauvage, obtenant ainsi un allergène mutant; à identifier un dérivé hypoallergénique dudit allergène de type sauvage par détection d'une déstabilisation ou d'un changement de la structure tridimensionnelle de l'allergène mutant par rapport à l'allergène de type sauvage par détermination des différences énergétiques entre l'allergène de type sauvage et l'allergène mutant pour obtenir un résultat Z.

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