Sequence preserved dna conversion

Details Sequence preserved dna conversion Sequence preserved dna conversion

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2741996

Described herein are inexpensive high throughput methods to convert a target single stranded DNA (ssDNA) such that each nucleotide (or base) adenine (A), thymine (T), guanine (G) and cytosine (C) is converted to a pre-determined oligonucleotide code, with the sequential order preserved in the converted ssDNA, or RNA. The method does not require the use of DNA polymerases during the cycles and involves the use of an oligonucleotide probe library with repeated cycles of ligation and cleavage. At each cycle, one or more nucleotides on one end (e.g., either the 5' end or the 3' end) of a target, e.g ssDNA, are cleaved and then ligated with the corresponding oligonucleotide code at the other end of the target ssDNA.

L'invention concerne des procédés peu coûteux à haut rendement permettant de modifier un ADN monocaténaire cible de sorte que chaque nucléotide (ou base) adénine (A), thymine (T), guanine (G) et cytosine (C) est transformé(e) en un code oligonucléotidique prédéterminé, l'ordre séquentiel étant conservé dans l'ADN monocaténaire converti ou l'ARN. Le procédé ne nécessite pas l'utilisation d'ADN polymérases pendant les cycles, et comprend l'utilisation d'une banque de sondes oligonucléotidiques avec des cycles répétés de ligature et de clivage. A chaque cycle, un ou plusieurs nucléotides se situant à une extrémité (p. ex., la terminaison 5' ou 3') d'un ADN monocaténaire cible, p. ex., sont clivés puis ligaturés, le code oligonucléotidique correspondant se situant à l'autre extrémité de l'ADN monocaténaire cible.

Affiliated with

Also associated with

Rating

Sequence preserved dna conversion does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sequence preserved dna conversion, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sequence preserved dna conversion will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1867863