Method for the storage of entomopathogenic nematodes

A - Human Necessities – 01 – N

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A01N 1/02 (2006.01) A01K 67/033 (2006.01) A01N 63/00 (2006.01) B65D 85/50 (2006.01)

Patent

CA 2144172

Long-term storage of infective third stage juveniles of nematodes belonging to the genera Steinernema or Heterorhabditis is effected by mixing together an aqueous cream of the nematodes and particles of a highly water-absorptive material. The water present in the mixture is such that the mixture, after equilibrating, has a water activity in the range of from 0.80 to 0.995 (prefer- ably 0.95 to 0.99). The preferred absorbent material is anhydrous polyacrylamide gel in the form of particles having an average mass of 0.01 gm. For optimal survival of the nematodes, the equilibrated mixture should be stored at a temperature in the range of from 1 °C to 30 °C, in an atmosphere which maintains the water activity of the mixture in the range of from 0.80 to 0.995.

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