Automated method and apparatus for embryonic stem cell culture

Details Automated method and apparatus for embryonic stem cell culture Automated method and apparatus for embryonic stem cell culture

C - Chemistry, Metallurgy

12

N

C12N 5/0735 (2010.01) C12N 5/071 (2010.01) C12M 3/00 (2006.01)

Patent

CA 2691793

The invention concerns methods for automated culture of embryonic stem cells (ESCs) such as human ESCs. In some aspects, methods of the invention employ optimized culture media and limited proteolytic treatment of cells to separate cell clusters for expansion. Automated systems for passage and expansion of ESCs are also provided.

L'invention concerne des procédés permettant une culture automatisée de cellules souches embryonnaires (ESC) telles que des ESC humaines. Dans certains aspects, les procédés de l'invention emploient des milieux de culture optimisés et un traitement protéolytique limité de cellules pour séparer les groupes de cellules pour expansion. L'invention concerne également des systèmes automatisés pour le passage et l'expansion d'ESC.

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