Method of controlled genetic transformation of an animal...

A - Human Necessities – 61 – K

Patent

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Details

A61K 47/48 (2006.01) A61D 1/02 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) A61M 5/42 (2006.01)

Patent

CA 2174210

The proposed method of controlled genetic transformation of a mammary gland involves: the introduction of genetic material into the animal's body in a soluble complex with carriers; the controlled delivery of genetic material by the said carriers to the target cell by specific receptor-mediated endocytosis, and whence to the cell nucleus by cytoplasm-nucleus transport. The said soluble complex is introduced into the animal's body via the mammary duct of the mammary gland. The proposed device for introducing genetic material into the mammary duct of an animal's mammary gland comprises a housing (1) with a depression (2) for accommodating the teat of the mammary gland, a central aperture (3) and an annular channel (4) at the base of the depression (2) which can communicate with a vacuum source for widening the teat sphincter. The central aperture (3) is used to introduce the device (6) through which the genetic material is introduced into the mammary duct.

L'invention concerne un procédé de transformation génétique maîtrisé d'une glande mammaire consistant à introduire une matière génétique dans le corps de l'animal dans un complexe soluble avec des excipients, à acheminer de manière maîtrisée la matière génétique à l'aide desdits excipients jusqu'à la cellule ciblée par endocytose induite par récepteur spécifique, et de là jusqu'au noyau cellulaire par transport du cytoplasme au noyau. On introduit ledit complexe soluble dans le corps de l'animal par l'intermédiaire des canaux galactophores de la glande mammaire. Le dispositif d'introduction de matière génétique de l'invention dans les conduits galactophores de la glande mammaire d'un animal comprend un corps (1) présentant une dépression (2) destinée à recevoir le trayon de la glande mammaire, une ouverture centrale (3) ainsi qu'un canal annulaire (4) situé au niveau de la base de la cavité (2) pouvant communiquer avec une source de vide destinée à élargir le sphincter du trayon. L'ouverture centrale (3) permet l'introduction du dispositif (6) à travers lequel on introduit la matière génétique dans les canaux galactophores.

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