C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01)
Patent
CA 2597482
The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a solid support, preferably magnetic beads, in the presence of an ethylene glycol multimer consisting of from 2 to 70 ethylene oxide monomers, preferably tetraethylene glycol, whereby soluble nucleic acid in said sample is bound to the surface of the support, and separating said support with bound nucleic acid from the sample. Kits for performance of the invention are also provided.
La présente invention concerne une méthode d'isolement d'acides nucléiques à partir d'un échantillon, ladite méthode comprenant la mise en contact dudit échantillon avec un support solide, préférentiellement des billes magnétiques, en présence d'un oligomère d'éthylène glycol constitué de 2 à 70 monomères d'oxyde d'éthylène, préférentiellement du tétraéthylène glycol, l'acide nucléique soluble au sein dudit échantillon se liant à la surface du support, ainsi que la séparation dudit support lié à l'acide nucléique d~avec l'échantillon. La présente invention décrit également des kits d'application de l'invention.
Invitrogen Dynal As
Mbm Intellectual Property Law Llp
LandOfFree
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