Methods and substances for recruiting therapeutic agents to...

A - Human Necessities – 61 – K

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A61K 47/48 (2006.01) A61K 9/127 (2006.01) A61K 39/44 (2006.01) C07K 16/30 (2006.01) C07K 16/46 (2006.01) C12N 11/06 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2062582

Disclosed is a method of using bifunctional binding molecules, such as two linked VH- VL single chain binding molecules, to recruit a therapeutic agent to a solid tissue site. The therapeutic agent is administered separately from the binding molecules and following the administration of a remover substance which aids in clearing free binding molecules in the circulation. In the preferred mode of the invention, the binding molecules have one specificity for antigens at the target site and one for the therapeutic agent. The binding molecules are administered and allowed time to approach a maximum concentration in the extravascular space. A remover substance, preferably a liposome conjugated with antibodies which are reactive with an antigenic epitope on the binding molecules, is then administered to remove excess binding molecules from the circulation and the extravascular space. A therapeutic agent, preferably a cytotoxic drug such as ricin A chain modified so as to enable it to enter the target cells once delivered to the target site, is then administered.

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