23s rna nucleotide fragment of the clhamydia genus bacteria,...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2262388

The invention concerns a single-stranded nucleotide fragment comprising at least a sequence of 12 contiguous nucleotide patterns, belonging to a 23S ribosome RNA strand of the species of the Chlamydia genus or to is complementary strand, said sequence being selected in any one of the following groups consisting of said RNA zones: Group I: 451-472, 542-570, 596-623, 731-756, 878-890, 996-1020, 1061-1094, 1123-1186, 1857-1880, 2234-2307, 2341-2370; Group II: 420-450, 473-514, 694-713, 756-790, 842-857, 927-937, 1231-1248, 1241-1319, 1371-1381, 1880-1895, 1943-1961, 2151-2182; Group III: 404-426, 436-457, 466-515, 683-722, 747-808, 817-863, 891-955, 1024-1055, 1208-1251, 1315-1350, 1407-1548, 1364-1388, 1576-1622, 1891-1918, 2148-218; the first number corresponding to the position of the first nucleotide of said zone relative to the 23S ribosome RNA nucleotide sequence of serotype A of Chlamydia trachomatis, SEQ ID NO:55, selected as reference sequence, and the second number corresponding to the position of the last nucleotide of said zone relative to this same reference sequence. The invention also concerns the uses of said fragment as probe, primer and in a reagent and method for detecting said bacteria.

L'invention concerne un fragment nucléotidique monocaténaire comprenant au moins une suite de 12 motifs nucléotidiques contigus, appartenant à un brin de l'ARN ribosomique 23S des espèces du genre Chlamydia ou à son complémentaire, cette suite étant choisie dans l'un quelconque des groupes suivants constitués par des zones dudit ARN: Groupe I: 451-472, 542-570, 596-623, 731-756, 878-890, 996-1020, 1061-1094, 1123-1186, 1857-1880, 2234-2307, 2341-2370; Groupe II: 420-450, 473-514, 694-713, 756-790, 842-857, 927-937, 1231-1248, 1241-1319, 1371-1381, 1880-1895, 1943-1961, 2151-2182; Groupe III: 404-426, 436-457, 466-515, 683-722, 747-808, 817-863, 891-955, 1024-1055, 1208-1251, 1315-1350, 1407-1548, 1364-1388, 1576-1622, 1891-1918, 2148-218; le premier numéro correspondant à la position du premier nucléotide de ladite zone par rapport à la séquence nucléotidique de l'ARN ribosomique 23S du sérovar A de Chlamydia trachomatis, SEQ ID NO:55, choisie comme séquence de référence, et le second numéro correspondant à la position du dernier nucléotide de ladite zone par rapport à cette même séquence de référence, et utilisations de ce fragment comme sonde, amorce ainsi que dans un réactif et procédé de détection desdites bactéries.

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