C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/04 (2006.01) C07K 1/14 (2006.01) C07K 14/39 (2006.01) C12N 1/06 (2006.01) C12N 15/81 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01) C12Q 1/26 (2006.01)
Patent
CA 2391522
A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form.
L'invention concerne un procédé permettant de libérer une protéine recherchée (POI) intercellulaire soluble ou associée à une membrane. Ce procédé comprenant les étapes consistant: à fournir une cellule comprenant une POI intercellulaire soluble ou associée à une membrane; à contacter la cellule avec une composition d'extraction de membrane; et à contraindre la cellule à libérer la POI dans des conditions suffisantes pour permettre la libération spécifique de la POI et dans une forme soluble.
Johansen Claus Lindvald
Kjaerulff Soren
Madrid Susan Mampusti
Pedersen Henrik
Poulsen Charlotte Horsmans
Danisco A/s
Gowling Lafleur Henderson Llp
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