Methods for rapid identification and quantitation of nucleic...

Details Methods for rapid identification and quantitation of nucleic... Methods for rapid identification and quantitation of nucleic...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2616281

There is a need for nucleic acid analysis which is both specific and rapid, and in which no nucleic acid sequencing is required. The present invention addresses this need, among others by providing a method of nucleic acid amplification of overlapping sub- segments of a nucleic acid followed by molecular mass measurement of resulting amplification products by mass spectrometry, and determination of the base compositions of the amplification products.

Il existe un besoin pour l'analyse d'acide nucléique qui est à la fois spécifique et rapide, dans laquelle aucun séquençage de l'acide nucléique n'est nécessaire. La présente invention répond à ce besoin, entre autres en fournissant un procédé d'amplification d'acide nucléique de sous-segments de recouvrement d'un acide nucléique suivi de mesure de masse moléculaire des produits d'amplification obtenus par la spectrométrie de masse, et la détermination des compositions de base des produits d'amplification.

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