C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/12 (2006.01) C07K 1/00 (2006.01) C12N 9/00 (2006.01) C12P 21/06 (2006.01) C12Q 1/48 (2006.01)
Patent
CA 2481409
The present invention relates to methods and compositions for providing purified thermostable enzymes, particularly thermostable DNA polymerases, that are free of exogenous detergents. The present invention also provides methods for providing such purified thermostable DNA polymerases to assays in an active form by adding one or more detergents. The present invention further provides compositions and kits comprising purified thermostable DNA polymerases for use in a variety of applications, including amplification and sequencing of nucleic acids.
La présente invention concerne des procédés et compositions pour fabriquer des enzymes thermostables purifiées, notamment des polymérases ADN thermostables, exemptes de détergents exogènes. La présente invention concerne aussi des procédés pour acheminer ces polymérases ADN thermostables purifiées dans des dosages sous forme active par l'ajout d'un ou de plusieurs détergents. La présente invention concerne aussi des compositions et des kits comprenant des polymérases ADN thermostables purifiées utilisées dans diverses applications, y compris l'amplification et le séquençage d'acides nucléiques.
Amersham Biosciences Corp
Fetherstonhaugh & Co.
Ge Healthcare Bio-Sciences Corp.
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