Parallel chromosomal stacking of traits in bacteria

C - Chemistry – Metallurgy – 12 – N

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C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12P 23/00 (2006.01)

Patent

CA 2509156

The invention describes a method for the stacking of traits in a recombination proficient host using a phage transduction system. The method makes use of a nucleic acid integration cassette that has homology to a specific site on a host chromosome for the insertion of genetic elements and the stacking of traits. Repetition of the method results in the stacking of traits on a single genetic element.

Cette invention se rapporte à un procédé d'empilement de caractères dans un hôte compétent de recombinaison au moyen d'un système de transduction par phage. Ledit procédé fait usage d'une cassette d'intégration d'acides nucléiques qui présente une homologie avec un site spécifique sur un chromosome hôte pour l'insertion d'éléments génétiques et l'empilement de caractères. La répétition du procédé permet d'obtenir l'empilement des caractères sur un élément génétique unique.

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