Image processing and analysis of array data

G - Physics – 06 – K

Patent

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G06K 9/00 (2006.01)

Patent

CA 2573978

Disclosed are methods of processing images, and in particular aligning and orienting grids from a fluorescent array of signal sources, by linking nearest neighbor sources to form a hexagon, and then using the hexagon lines for alignment and orientation of the grid. Also disclosed are methods and algorithms for aligning image with grid and correcting for signal beads which are smaller than a grid field and the shift in a grid field. These methods can be used where the signal sources are fluorescent images from a microarray. Also disclosed are methods of automated watershed clustering following transformation.

L'invention porte sur des procédés de traitement d'images et, notamment, sur des procédés de calage et d'orientation de grille à partir d'une matrice fluorescente de sources du signal, en reliant les sources voisines les plus proches de façon à former un hexagone et en utilisant ensuite les lignes de l'hexagone pour caler et orienter la grille. L'invention porte également sur des procédés et sur des algorithmes de calage d'images sur la grille et sur des procédés de correction tenant compte, d'une part, des dimensions des points des billes n'occupant pas entièrement une zone de grille et, d'autre part, du glissement affectant une zone de grille. Ces procédés conviennent particulièrement dans le cas où les sources du signal sont des images fluorescentes provenant de microréseaux. L'invention porte également sur des procédés de groupage automatisé, par collecte "à l'épuisette", après transformation.

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