Intrinsic factor - horse radish peroxidase conjugates

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/96 (2006.01) C12N 9/08 (2006.01) G01N 33/535 (2006.01) G01N 33/82 (2006.01)

Patent

CA 2104413

This invention presents a non-isotopic competitive assay for Vitamin B12 (B12), utilizing intrinsic factor (IF) labelled with horse radish peroxidase (HRP). The conjugation is accomplished by oxidizing surface carbohydrates on the HRP, and reacting this with IF.

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