C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/24 (2006.01) C12N 15/12 (2006.01) C12N 15/56 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2281830
A method for extraction of heterologous protein from plant seed comprises extracting the germ portion of the seed and extracting and purifying the protein from the germ. Enhanced expression in the germ is provided, and allows for improved efficiency in production, and cost savings. Directing expression to the germ portion further increases expression levels of the protein. The ubiquitin promoter preferentially directs expression to the germ portion of plant seed.
On décrit une méthode d'extraction d'une protéine hétérologue à partir de graines, qui consiste à extraire la partie germinale de la graine puis à extraire et purifier la protéine ainsi obtenue. Cela donne une expression améliorée dans le germe, une efficacité de production accrue et une économie de coûts. L'orientation de l'expression vers la partie germinale permet en outre d'accroître les niveaux d'expression de la protéine. De préférence, l'ubiquitine promotrice oriente l'expression vers la partie germinale de la graine.
Prodigene Inc.
Torys Llp
LandOfFree
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