Process for massive conversion of clostridia into...

Details Process for massive conversion of clostridia into... Process for massive conversion of clostridia into...

C - Chemistry, Metallurgy

12

N

C12N 1/20 (2006.01) C12N 1/36 (2006.01) C12N 3/00 (2006.01)

Patent

CA 2109808

2109808 9220782 PCTABS00017 A method is disclosed for producing Clostridium which produce solvents, enzymes, antibiotics, toxic proteins or spores. Cell elongation to a critical length of at least about 3X is induced by serial subculturing under controlled conditions to effect synchronization of growth in the number of cells and their effective mass and to produce a substantially homogenous cell population. At least about 0,01M of a divalent cation such as calcium is added to the synchronized cells of critical length to stabilize the cells. Spore formation may be permitted to continue until substantially the entire cell population constitutes refractile mature free spores, which may be harvested.

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