Complete chemical and enzymatic treatment of phosphorylated...

Details Complete chemical and enzymatic treatment of phosphorylated... Complete chemical and enzymatic treatment of phosphorylated...

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2507117

A simple and quick protocol for chemical treatment, enzymatic digestion and subsequent identification of proteins on protein chip arrays is disclosed. The chemical treatment comprises denaturation, reduction and alkylation, while enzymatic digestion encompasses deglycosylation, dephosphorylation, and digestion by various proteases. All reactions are carried out on-chip in a sequential fashion. Subsequent peptide mass fingerprinting allows the identification of specific peptides. The method of the present invention can be applied to the analysis of biological samples such as urine to identify specific biomarkers. The methods of the present invention allow complete on-chip treatment, which can be used for rapid protein identification and structural characterization.

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