Reduced temperature dna denaturation using glycerol

Details Reduced temperature dna denaturation using glycerol Reduced temperature dna denaturation using glycerol

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2159206

Method for determining the nucleotide base sequence of a double-stranded closed circular DNA. The method includes heating a solution which includes the DNA in the presence of at least 20 % glycerol and/or ethylene glycol to at least 80 °C to provide denatured DNA, and then sequencing the denatured DNA.

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