Method for expanding hematopoietic stem cells

Details Method for expanding hematopoietic stem cells Method for expanding hematopoietic stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/0789 (2010.01) C12N 5/071 (2010.01)

Patent

CA 2465001

A method of culturing Lin-negative or weakly positive cells characterized by culturing in the presence of macrophage colony stimulating factor (M-CSF); a method of amplifying hematopoietic stem cells characterized by culturing Lin- negative or weakly positive cells in the presence of M-CSF; a hematopoietic stem cell mass obtained by the amplification method; a kit for culturing Lin- negative or weakly positive cells containing M-CSF; and a hematopoietic stem cell amplification promoter containing M-CSF.

L'invention porte: sur un procédé de culture de cellules Lin-négatives ou faiblement positives en présence du facteur de stimulation de la colonie de macrophages (M-CSF); sur un procédé d'amplification de cellules hématopoïétiques souches par culture de cellules Lin-négatives ou faiblement positives en présence du facteur M-CSF; sur une masse de cellules hématopoïétiques souches obtenue par un procédé d'amplification; sur une trousse de culture de cellules Lin-négatives ou faiblement positives en présence du facteur M-CSF; et sur un promoteur d'amplification de cellules hématopoïétiques souches contenant le facteur M-CSF.

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