C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0735 (2010.01) C12N 5/00 (2006.01) C12N 5/02 (2006.01) C12N 15/00 (2006.01) A61K 35/12 (2006.01)
Patent
CA 2307807
Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate- derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from the group consisting of feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from the group consisting of nucleosides and a pyruvate salt.
L'invention concerne des procédés et des matières utiles pour la culture de cellules souches primordiales de primate. Dans un mode de réalisation, on prévoit un milieu de culture de cellules utile pour la croissance de cellules souches primordiales de primate dans un état sensiblement indifférencié, qui comporte un milieu basique à pression osmotique faible, faiblement endotoxinique, qui sert de support à la croissance de cellules souches primordiales de primate. Le milieu basique est combiné avec un sérum nutritif qui sert de support à la croissance de cellules souches primordiales de primate, et avec un substrat sélectionné dans le groupe constitué par des cellules nourricières et un constituant de matrice extracellulaire obtenu à partir de cellules nourricières. Le milieu comporte en outre des acides aminés non essentiels et un premier facteur de croissance sélectionné dans le groupe constitué par des nucléosides et un sel de pyruvate.
Bodnar Andrea G.
Chiu Choy-Pik
Gold Joseph D.
Inokuma Margaret
Murai James T.
Geron Corporation
Smart & Biggar
LandOfFree
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