Detection of pathogenic microorganisms and their...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/08 (2006.01) C12M 1/18 (2006.01) C12Q 1/04 (2006.01) C12Q 1/18 (2006.01) C12Q 1/20 (2006.01) G01N 21/63 (2006.01)

Patent

CA 2305789

A multicompartment assay device comprising at least one compartment comprising a viable organism control medium capable of sustaining growth of total microbial organisms; at least one compartment comprising a medium capable of selectively sustaining growth of target microbial organisms; and, at least one compartment comprising an antimicrobial susceptibility interpretation medium. Also, a method of detecting the presence of target microbial microorganisms in a biological sample and of simultaneously determining the susceptibility of such microorganisms to antimicrobial agents by use of such a device.

L'invention porte sur un dispositif de dosage à plusieurs compartiments dont: au moins un compartiment comprend un milieu de régulation de micro-organismes viables capable de supporter la prolifération de la totalité des micro-organismes microbiens; au moins un compartiment comprenant un milieu capable de supporter sélectivement la prolifération de micro-organismes microbiens cibles; et au moins un compartiment comprenant un milieu d'interprétation de la sensibilité aux antimicrobiens. L'invention porte également sur un procédé de détection de micro-organismes microbiens cibles dans un échantillon biologique et de détermination simultanée de la sensibilité de ces micro-organismes à des agents antimicrobiens au moyen de ce dispositif.

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