C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/16 (2006.01) C12N 9/18 (2006.01) C12N 15/55 (2006.01) C12P 7/62 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2219959
The present invention relates to polyhydroxyalkanoate depolymerase having a N-terminal fragment of the amino acid sequence of SEQ ID NO: 1 and having a molecular weight of about 33,000 as determined by SDS polyacrylamide gel electrophoresis; a process for producing polyhydroxyalkanoate depolymerase, comprising culturing in a medium a microorganism which belongs to the genus Corynebacterium and has the ability to produce the polyhydroxyalkanoate depolymerase and recovering the polyhydroxyalkanoate depolymerase from the resulting culture; and a process for producing polyhydroxyalkanoate depolymerase, comprising culturing in a medium a transformant transformed with a recombinant vector containing a gene of the polyhydroxyalkanoate depolymerase and recovering the polyhydroxyalkanoate depolymerase from the resulting culture. According to the present invention, there can be provided novel PHA depolymerase with the activity of decomposing .omega.-hydroxyalkanoates, particularly 4HB homopolyester or copolymerized polyesters containing said homopolyester, and a process of efficiently producing the enzyme.
La présente invention concerne la polyhydroxyalcanoate-dépolymérase ayant un fragment N-terminal de la séquence d'acides aminés SEQ ID NO : 1 et ayant un poids moléculaire d'environ 33 000 d'après l'électrophorèse sur gel de polyacrylamide en présence de SDS, un procédé pour produire de la polyhydroxyalcanoate-dépolymérase, comprenant la culture dans un milieu d'un microorganisme qui appartient au genre Corynebacterium et qui a la capacité de synthétiser la polyhydroxyalcanoate-dépolymérase et la récupération de la polyhydroxyalcanoate-dépolymérase de la culture obtenue; et un procédé pour produire de la polyhydroxyalcanoate-dépolymérase, comprenant la culture dans un milieu d'un transformant transformé à l'aide d'un vecteur recombinant contenant un gène de la polyhydroxyalcanoate-dépolymérase et la récupération de la polyhydroxyalcanoate-dépolymérase de la culture obtenue. Conformément à la présente invention, on peut fournir une nouvelle polyhydroxyalcanoate-dépolymérase qui a pour effet de décomposer les oméga-hydroxyalcanoates, particulièrement l'homopolyester 4-hydroxybutyrique ou les polyesters copolymérisés contenant ledit homopolyester, et un procédé efficace de production de l'enzyme.
Matsunobu Toshio
Saito Yuji
Shibayama Masako
Takebe Hidehi
Hill & Schumacher
Meiji Seika Kaisha Ltd.
Taisei Corporation
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