Method and kit for determining severe inflammatory reactions...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A61K 38/17 (2006.01) C07K 14/435 (2006.01) C07K 16/18 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2309387

Mosquito extract consisting essentially of antigens solely to allergens in mosquito saliva. Isolated and purified recombinant mosquito saliva antigens for use in skin tests, immunoassays and immunotherapy for allergic reactions to mosquito bites. A method for skin testing and determining the undertaking of immunotherapy includes the steps of administrating the isolated and purified mosquito salivary antigens to a patient and then recognizing skin reaction thereto is a positive indication of needing therapy. A kit and an immunoassay are also provided for measurement of mosquito salivary allergen specific IgE and IgG wherein the assay includes the isolated and purified recombinant mosquito allergens as a substrate to which allergen specific IgE and IgG binds. Antibodies directed against the recombinant salivary allergens of mosquitoes are also disclosed.

Cette invention, qui a trait à une substance extraite du moustique consistant principalement en des antigènes spécifiques, uniquement, d'allergènes se trouvant dans la salive du moustique, concerne également des antigènes de recombinaison de la salive du moustique, isolés et purifiés. la méthode d'épreuve cutanée et de détermination de l'immunothérapie à mettre en oeuvre consiste à administrer au patient les antigènes salivaires de moustique isolés et purifiés, la réaction cutanée évaluée constituant une indication positive de la nécessité d'une thérapie. L'invention porte également sur un nécessaire et sur un dosage immunologique aux fins de la mesure des immunoglobulines E et G spécifiques de l'allergène salivaire du moustique. Le dosage utilise les allergènes salivaires de moustique de recombinaison, isolés et purifiés, comme substrat sur lequel se fixent les immunoglobulines E et G spécifiques de l'allergène. Cette invention a également trait à des anticorps opposés aux allergènes salivaires de recombinaison de moustiques

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