Process and nutrient medium for growing microorganism

Details Process and nutrient medium for growing microorganism Process and nutrient medium for growing microorganism

C - Chemistry, Metallurgy

12

N

195/123, 195/43

C12N 9/78 (2006.01) C12N 1/20 (2006.01)

Patent

CA 1139695

ABSTRACT OF THE DISCLOSURE A fermentation process and improved aqueous nu- trient medium is used for the production of urease-free creatinine iminohydrolase from an aerobic soil microorganism. In the process, a fresh sample of the microorganism grown on a creatinine-containing maintenance medium is transferred in a microbial growth medium to grow the microorganism, the growing microorganism in then transferred to a production medium to produce microorganism in which creatinine imino- hydrolase production has been induced, and the desired enzyme is then extracted from the microorganism. An im- proved aqueous nutrient medium for use as the aforementioned production medium is disclosed.

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