Multi-spectral segmentation for image analysis

G - Physics – 06 – T

Patent

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G06T 7/40 (2006.01) G06T 5/00 (2006.01)

Patent

CA 2227224

A method for segmenting spectrally-resolved images. The first step comprises acquisition of three images of the same micrographic scene. Each image is obtained using a different narrow band-pass optical filter which has the effect of selecting a narrow band of optical wavelengths associated with distinguishing absorption peaks in the stain spectra. The choice of optical wavelength bands is guided by the degree of separation afforded by these peaks when used to distinguish the different types of cellular material on the slide surface. By combining these images in a particular fashion, it is possible to achieve a high degree of success in separating the cervical cell from the background and the nuclei from the cytoplasm.

L'invention a pour objet un procédé pour segmenter des images à résolution spectrale. La première étape consiste à acquérir trois images de la même scène micrographique. Chaque image est obtenue à l'aide d'un filtre optique passe-bande étroit différent, qui a pour effet de sélectionner une bande étroite de longueurs d'ondes optiques associées aux pics d'absorption différents dans les spectres du colorant. Le choix des bandes de longueurs d'ondes optiques est guidé par le degré de séparation assuré par ces pics, lors de leur utilisation pour différencier les différents types de matériau cellulaire sur la surface de la lamelle porte-objet. En combinant ces images d'une manière particulière, on peut séparer, de manière très satisfaisante, la cellule cervicale du fond et les noyaux du cytoplasme.

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