C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/00 (2006.01) C07H 21/02 (2006.01) C12N 9/12 (2006.01) C12P 19/34 (2006.01) C12Q 1/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2508554
The mutant T7, T3 and SP6 RNA polymerase enzymes have a number of novel properties that would be desirable for those who wish to use the phage RNAP to synthesize homogeneous RNA products. These includes: reduced synthesis of aberrant products on templates that have protruding 3~ ends in the non- template strand, decreased addition of a non-templated nucleotide to the 37 end of transcript; increased yields of products on templates that termioate in a G:c-rich context. The mutations include deletions at positions 172 and 173 of the T7 RNA polymerase, deletions at positions 140-143 of the SP6 RNA polymerase and deletions at positions 173 and 174 of the T3 RNA polymerase.
La présente invention a trait à des procédés et des matériaux améliorés pour réduire la production de produits aberrants lors de la synthèse d'ARN et la production de protéines.
Kukarin Alexander
Mcallister William T.
Shapiro Cohen
The Research Foundation Of State University Of New York
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