Method of preparing pluripotent stem cells

Details Method of preparing pluripotent stem cells Method of preparing pluripotent stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01)

Patent

CA 2758299

The invention relates to biotechnology, and particularly to the preparation of pluripotent stem cells. The method involves introduction into umbilical cord and placental stem cells of RNA with at least one sequence which ensures the transition of cells to the pluripotent state. The method enables to effectively prepare pluripotent stem cells from the cells of mammalian placenta and umbilical cord which have not yet acquired somatic mutations, which reduces the risk of oncogenesis and other adverse effects of reprogramming.

L'invention concerne la biotechnologie, à savoir la production de cellules pluripotentes. La méthode consiste à introduire l'ARN dans les cellules d'un complexe ombilical / placentaire, qui possède au moins une séquence qui prévoit le passage des cellules dans un état pluripotent. La méthode permet de produire efficacement des cellules pluripotentes à partir des cellules d'un complexe ombilical / placentaire qui n'ont pas encore subi de mutations somatiques, ce qui réduit la probabilité de l'oncogenèse et d'autres conséquences négatives de la reprogrammation.

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