Composite membranes for assaying biomolecules

G - Physics – 01 – N

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Details

G01N 33/544 (2006.01) B01D 69/02 (2006.01) B01D 69/10 (2006.01) B01D 71/56 (2006.01) C12Q 1/68 (2006.01) B01L 3/00 (2006.01)

Patent

CA 2399163

Disclosed are smooth surfaced porous membranes having one or more advantages such as low autofluorescence, thermal-cyclability, especially under humid conditions, and three-dimensional binding capacity. The membrane can be free- standing or, preferably in combination with a support as in a composite membrane. The present invention provides a composite membrane comprising a porous polymer layer disposed on a support. The present invention further provides devices such as microarray devices comprising the composite for the analysis of biomaterials such as nucleic acids.

L'invention concerne des membranes poreuses à surface lisse présentant un ou plusieurs avantages, tels qu'une faible autofluorescence, une aptitude au cyclage thermique, notamment dans des conditions humides, et une capacité de liaison tridimensionnelle. Cette membrane peut être isolée ou, de préférence, utilisée en combinaison avec un support comme dans le cas d'une membrane composite. La présente invention concerne également une membrane composite comprenant une couche polymérique poreuse disposée sur un support. Elle se rapporte en outre à des dispositifs, tels que des dispositifs à jeux ordonnés de microéchantillons, comprenant ce composite et permettant d'analyser des matières biologiques, et notamment des acides nucléiques.

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