Microinjection of cryoprotectants for preservation of cells

Details Microinjection of cryoprotectants for preservation of cells Microinjection of cryoprotectants for preservation of cells

A - Human Necessities

01

N

A01N 1/02 (2006.01) A01N 37/02 (2006.01)

Patent

CA 2409102

A preservation method for biological material having cell membranes includes microinjecting the cells with sugar; preparing the cells for storage; storing the biological material; and recovering the stored biological material from storage. Carbohydrate sugars such as trehalose, sucrose, fructose, dextran, and raffinose, may be used as bio-protective agents.

L'invention concerne un procédé permettant la conservation de matériel biologique comportant des membranes cellulaires. Ce procédé consiste à introduire du sucre dans les cellules par micro-injection, à préparer ces cellules pour l'entreposage, à entreposer le matériel biologique, et à sortir le matériel biologique entreposé de l'entreposage. Les hydrates de carbone tels que le tréhalose, le saccharose, le dextrane et le raffinose peuvent être utilisés en tant qu'agents bioprotecteurs.

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