Gene expression profiling for predicting the survivability...

Details Gene expression profiling for predicting the survivability... Gene expression profiling for predicting the survivability...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2730277

A method is provided in various embodiments for deter-mining a profile data set for predicting the survivability of a subject with prostate cancer based on a sample from the subject, wherein the sample provides a source of RNAs. The method includes using amplification un-der measurement conditions that are substantially repeatable for measur-ing the amount of RNA corresponding to at least 1 constituent from Table 1. Alternatively, the method uses electrophoresis or immunohistochem-istry for measuring the mount of protein corresponding to at least 1 con-stituent from Table 20. The profile data set comprises the measure of each constituent.

L'invention porte sur un procédé dans divers modes de réalisation pour la détermination d'un ensemble de données de profil pour la prédiction de la survie d'un sujet atteint d'un cancer de la prostate sur la base d'un échantillon provenant du sujet, l'échantillon fournissant une source d'ARN. Le procédé comprend l'utilisation d'une amplification dans des conditions de mesure qui sont pour l'essentiel répétables pour la mesure de la quantité d'ARN correspondant à au moins un constituant du tableau 1. En variante, le procédé utilise l'électrophorèse ou l'immunohistochimie pour la mesure de la quantité de protéine correspondant à au moins un constituant du tableau 20. L'ensemble de données de profil comprennent la mesure de chaque constituant.

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